Date Published:
05/2004

Publication Type:
Journal Article

Authors:

K.A. Carlson
J. Limoges
G.D. Pohlman
L.Y. Poluektova
D. Langford
E. Masliah
T. Ikezu
H.E. Gendelman

Secondary:
Journal of Neuroimmunology

Volume:
150

Pagination:
186-98

Issue:
1-2

URL:
https://pubmed.ncbi.nlm.nih.gov/

Keywords:
Cells;Cultured;DNA-Binding Proteins;Encephalitis;External;Organ Specificity;Transcription Factors;Viral

Abstract:
<p>OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.</p>