OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis
Title | OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis |
Publication Type | Journal Article |
Year of Publication | 2004 |
Authors | Carlson, KA, Limoges, J, Pohlman, GD, Poluektova, LY, Langford, D, Masliah, E, Ikezu, T, Gendelman, HE |
Journal | Journal of Neuroimmunology |
Volume | 150 |
Issue | 1-2 |
Pagination | 186-98 |
Date Published | 05/2004 |
Keywords | Cells, Cultured, DNA-Binding Proteins, Encephalitis, External, Organ Specificity, Transcription Factors, Viral |
Abstract | OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis. |
URL | http://www.ncbi.nlm.nih.gov/pubmed/15081264 |